SESSION RECORDINGS
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MONDAY, September 27, 2021
WELCOME and OPENING
SFB and GEM presidents: Eric Ennifar and Isabelle Mus-Veteau
Hosts: Isabel ALVES, CBMN, Bordeaux and Christine GRAUBY-HEYWANG, LOMA, Bordeaux
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SESSION 1 / Part 1: Protein-Lipid Interaction - Membrane Active Peptides
Chairman: Burkhard Bechinger
Speakers:
9:15 - 9:55 Invited Speaker: Karin Thevissen (Belgium) “Membrane-interacting antifungal plant defensins”
9:55 - 10:15 Enrico Semeraro (Austria) “Bridging the activity of lactoferricin derivatives in E. coli and lipid-only membranes: partitioning and kinetics”
10:15 - 10:35 Claudia Herrera Leon (France) “The importance of motifs in anticancer peptides detected by sequence alignment: a biophysical study”
10:35 - 10:55 Alexandre Chenal (France) “CyaA toxin cell invasion involves membrane-active and calmodulin-binding properties of its translocation domain”
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11:05 - 11:45 PM (CEST) TALK 1 «SFB Young Investigator Award»
Chairman: Eric Ennifar
Speaker: Alexandra Colin (France) “Reconstitution of actin dynamic steady state in cell-sized confinement”
11:50 - 12:30 PM (CEST) TALK 2 «GEM Best PhD Thesis Award»
Chairwoman: Isabelle Mus-Veteau
Speaker: Thibaud Dieudonné (France) “Functional and structural characterization of lipid flippases: the yeast Drs2/Cdc50 and the disease-related human ATP8B1/CDC50A complexes”
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Session 1 / Part 2: Protein-lipid interaction - Membrane proteins
Chairwoman: Isabelle Mus-Veteau
13:30 - 14:10 Invited Speaker: Rumiana Dimova (Germany) “Membrane remodeling in artificial cells”
14:10 - 14:30 Vincent Nieto (France) “Distribution of proteins in nascent lipid droplets”
14:30 - 14:50 Evgeniy Salnikov (France) “Solid-state NMR investigations of the MHC II transmembrane domains: topological equilibria and lipid interactions”
14:50 - 15:10 Matthias Pöhln (Germany) “Lipid nanodiscs as a tool to unlock membrane dynamics”
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Industrial Scientific Talks
Chairwoman: Isabel Alves
15:15 - 15:35 Kalthoum Ben M’barek (Lumicks, Netherlands) “Correlative single-molecule force-FRET measurements of DNA hairpin conformational dynamics”
15:35 - 15:55 Amandine Gontier (Dynamic Biosensors, Germany) “heliX®: Next-Generation Modular Biosensor for Interaction and Conformation Analysis”
15:55 - 16:15 Henrik Jensen (Fidabio, Denmark) “Flow induced dispersion analysis: A new technology for biophysical characterization of proteins, their interactions and ability to engage in liquid-liquid phase separation”
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Monday, Sept 27, 2021
Flash Poster Session (1)
3 min flash poster followed by Q&A in individual breakout rooms
Chairwoman: Rim Baccouch
Poster Presenters:
1.1. Rima Budvytyte, 1.2. Adrien Gebus, 1.3. Evelina Jankaitytė, 1.4. Marija Jankunec, 1.5. Lucie Khemtemourian, 1.6. Javier Lizarrondo, 1.7. Janina Louisa Nandy, 1.8. Tibor Páli, 1.10. Fabio Perissinotto, 1.11. Francisco Ramos, 1.12. Caterina Ricci, 1.13. Adrien Royet, 1.14. Ahmad Saad
Flash Poster Videos only
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TUESDAY: September 28, 2021
SESSION 2: Mechanobiology and cell motility
Chairman: Guillaume Lenoir
9:00 - 9:40 Invited Speaker: Sandrine Etienne-Manneville (Paris) “Microtubules regulate mechanosensitive cell migration”
9:40 - 10:00 Maria Alai (Germany) “An integrative structural biology approach to tackle membrane remodeling”
10:00 - 10:20 Margaux Lecacheur (France) “Mechanical activation of the collagen receptors DDR1 and DDR2 promotes melanoma aggressiveness through YAP pathway”
10:20 - 10:40 Cédric Montigny (France) “Sarcolipin alters SERCA1a interdomain communication by impairing both calcium and ATP binding”
10:40 - 11:00 Yosuke Senju (Japan) “Regulation of actin dynamics by phosphoinositides”
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Tuesday, Sept 28, 2021
SESSION 3: Glycobiology, Sweets and Membranes
Chairman: Christophe Moreau
11:10 - 11:50 Invited Speaker: Rabia Sadir (France) “Heparan Sulfate/Protein interactions: structural and functional consequences”
11:50 - 12:10 Antoine Loquet (France) “Polysaccharides and lipid organization in capsule and surface of pathogen cells in their intact form revealed by new solid-state NMR approaches.”
12:10 - 12:30 Larissa Socrier (Germany) “The structure of Gb3 receptors influences their partitioning in membranes: impact on bacterial Shiga Toxin B insertion in lipid monolayers.”
12:30 - 12:50 Yadira Hervis (France) “Role of a glycosaminoglycan-binding motif in the internalization of Engrailed-2 homeoprotein”
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Tuesday, Sept 28, 2021
SESSION 4: Membrane protein structure and dynamics
Chairs: Luca Monticelli and Christophe Moreau
13:50 - 14:30 Invited Speaker: Guido Pintacuda (France) “Fast magic-angle spinning NMR of membrane proteins”
14:30 - 14:50 Hélène Demené (France) “Activation pathway of the V2 vasopressin GPCR by combined use of cryoEM and NMR”
14:50 - 15:10 Rim Baccouch (France) “The impact of membrane lipid poly-unsaturation on Dopamine D2 receptor internalization pathway”
15:10 - 15:30 Dolores Garcia Fernandez (France) “The ligand-bound state of a G protein-coupled receptor stabilizes the interaction of functional cholesterol molecules”
15:30 - 15:50 Martin Caffrey (Ireland) “Structural insights into the reaction mechanism of a new bacterial lipoprotein processing enzyme”
15:50 - 16:10 Marlene Martinho (France) “The assembly of Aquaporin Z in Escherichia coli: an SDSL and EPR spectroscopy study”
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Flash Poster Session (2)
3 min flash poster followed by Q&A in individual breakout rooms
Chairwomen: Esther Boyer and Margaux Lustig
Poster Presenters:
2.15. Noha AL-QATABI, 2.16. Sarah Benamara, 2.17. Esther Boyer, 2.18. Alexandre Ciaccafava, 2.19. Sophie Combet, 2.20. Giacomo Corucci, 2.21. Nesrine Debez, 2.22. Eric Dufourc, 2.23. Frédérique Favier, 2.24. Florent Laydevant, 2.25. Mathieu Letrou, 2.26. Alexis Lode, 2.27. Margaux Lustig, 2.28. Gilmar Salgado, 2.29. Ana Filipa Santos Seica, 2.30. Pierre Soule / Cyril Castel, 2.31. Manuela Zoonens / Babtiste Michon
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Wednesday, Sept 29, 2021
SESSION 5: New Technologies
Chairwoman: Véronique Vié
Speakers:
9:00 - 9:40 Invited Speaker: Tilman Schäffer (Germany) “Scanning ion conductance microscopy (SICM) for measuring dynamics and mechanics of cells”
9:40 -10:00 Robert Quast (France) “Single molecule FRET reveals full activation of metabotropic glutamate receptor 2 by allosteric modulation”
10:00 -10:20 Estelle Rascol (France) “Biophysical methods to characterize ligand binding affinities to GPCRs in non-purified cell membrane lysates”
10:20 -10:40 Pierre Joseph (France) “A microfluidic aspiration pipette to measure the bending and stretching moduli of model membranes”
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Wednesday, Sept 29, 2021
SESSION 6: New Frontiers in Bioimaging
Chairman: Emmanuel Margeat
Speakers:
10:50 - 11:30 Invited speaker - Aurélie Bertin (France) “Behavior of septins at the membrane using cell-free bottom up assays”
11:30 - 11:50 Anthony Vial (France) “Structural analysis of nuclear pore complex basket by AFM/Fluorescence correlative microscopy”
11:50 - 12:10 Mouna Abdesselem (France) “Exploration of Protein Complexes and Microdomains on the Endoplasmic Reticulum Membrane with FRET-FLIM”
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Wednesday, Sept 29, 2021
SESSION 7: Mass spectrometry approaches in membrane protein studies
Chairwoman: Sarah Cianferani
Speakers:
13:10 - 13:50 Invited Speaker: Perdita Barran(UK) “Light footprinting proteins”
13:50 - 14.30 Invited Speaker: Leonhard Urner(UK) “Oligoglycerol Detergents for Native Mass Spectrometry of Membrane Proteins”
14:30 - 14:50 Cherine Bechara (France) “Molecular insights into mechanisms of GPCR hijacking by S. aureus”
14:50 - 15:10 Simon Drescher (Germany) “A Diazirine‐Modified Membrane Lipid to Study Peptide/Lipid Interactions –Chances and Challenges”
15:10 - 15:30 Margot Di Cesare (France) “Conformational changes of a bacterial ABC multidrug efflux pump, BmrA, witnessed through Hydrogen-Deuterium Exchange coupled to Mass Spectrometry”
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SESSION 8: Disordered protein domains and liquid-liquid phase separation
Chairman: Ludger Johannes and John Hjort Ipsen
Speakers
15:35 - 15:55 Lorena Redondo-Morata (France) “Non-equilibrium dynamics and nanomechanics of lipid membranes”
15:55 - 16:15 Ester Canepa (Italy) “Amphiphilic gold nanoparticles perturb lipid phase separation in biomimetic cell membranes”
16:25 - 17:05 Invited Speaker: Jeanne Stachowiak (USA) “Disordered protein networks as synergistic drivers of membrane traffic”
17:05 - 17:25 Moritz Frewein (France) “Interdigitation-Induced Interleaflet Coupling in Asymmetric Liposomes”
17:25 - 18:05 Invited Speaker: Alf Honigmann (Germany) “Role of protein phase separation for the assembly of tight junctions”
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THURSDAY: September 30, 2021
Talk: “MOSBRI: Your European partner for molecular-scale biophysical characterization”
Chairwoman: Christine Grauby-Heywang
Speaker: Patrick England (France)
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SESSION 9: Integrative approaches in molecular modeling
Chairman: Marc Baaden
Speakers:
9:20 - 10:00 Invited Speaker: Jana Selent (Spain) “Deciphering the functionality of G protein-coupled receptors via computation and experiment”
10:00 - 10:20 Meline Simsir (France) “Understanding Ptch1 dynamics and interactions: from antibioresistance to chemotherapy resistance”
10:30 - 10:50 Cyril Favard (France) “How HIV-1 Gag self-assembly remodel the plasma membrane: From in silico to in cellulo”
10:50 - 11:10 Stéphane Abel (France) “Combining experimental (SEC-MALLS and SEC-SAXS) and atomistic MD simulation approaches to investigate the structure of large membrane protein complex: A comparative study of the membrane receptor ShuA in DDM micelles and native-like membrane.”
11:10 - 11:30 Marius Trollmann (Germany) “Characterization of a Lipid Nanoparticle for mRNA Delivery using Molecular Dynamics”
11:30 - 11:50 Robert Vacha (Czech Republic) “Enhanced translocation of amphiphilic peptides across membranes by transmembrane proteins”
11:50 - 12:10 Astrid Brandner (France) “Don’t forget the flow: Multi-scale simulations methods for biological membrane systems”
Move forward to 00:20 min for Session 9
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Flash Poster Session (3)
3 min flash poster followed by individual breakout rooms
Chairwoman: Larissa Socrier
Poster Presenters
3.32. Krishna Batchu, 3.33. Mehdi Benmameri, 3.34. Astrid Brandner, 3.35. Marine Cosset, 3.36. Nicolas Destainville, 3.37. Christine Doucet, 3.38. Maria Hoernke, 3.39. Stephane Jedele, 3.40. Houssem Kahli, 3.41. André Koernig, 3.42. Elodie Lafargue, 3.43. Simon Legood, 3.45. Sergio Alejandro Poveda Cuevas, 3.46. Nely Rodriguez-Moraga, 3.47. Afroditi Vaitsopoulou, 3.48. Francisco Ramos, 3.49. Victor Galileo Almendro Vedia, 3.50. Amandine Gontier
Flash Poster Videos only. Enter passcode.
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POSTER PRIZES, AWARDS & CLOSING
Moderators: Isabel ALVES, CBMN, Bordeaux and Christine GRAUBY-HEYWANG, LOMA, Bordeaux
